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Western blot Cells were harvested and proteins were extracted using cell lysis buffer supplemented with 0.3% phenylmethylsulfonyl fluoride and proteinase and phosphatase inhibitors

Glutathione is a potent free radical scavenger, Alan says, which means it can hunt down free radicals before they cause oxidative damage and oxidative stress

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When GHK-Cu goes into solution it takes on a distinct blue tint from the copper complex, which is a normal sign of a correctly reconstituted vial

The ratio is useful for controlled comparisons but is not a complete thermodynamic description of redox state