For the extraction of mRNA, tissue samples were homogenized in TRI Reagent (Molecular Research Center) using a TissueLyser II system (Qiagen) and then chloroform extracted, precipitated using isopropanol, washed with 75% ethanol, and reconstituted with DEPC-treated water
By binding to regional receptors in the gastrointestinal tract, GLP-1s alter gut motility in different areas of your intestines, affecting the physical contractions which push food (and waste) through your body
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Roostaee M, Barzegar M, Sahari MA, Rafiee Z