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This assay is an alternative to the LDH cytotoxicity assay which measures the activity of lactate dehydrogenase (LDH) released upon rupture of cell membrane integrity

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The mitochondrial steroidogenic acute regulatory protein (StAR) mediates cholesterol transport to the inner mitochondrial membrane, enabling pregnenolone synthesis by P450scc, a process finely regulated by gonadotropins such as FSH and LH [44, 45]

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