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Kinetic parameters of purified recombinant enzymes were determined from assays containing varying concentrations of arogenate (39.1 M5 mM) or prephenate (46.9 M6 mM) substrate, 0.8 mM of the preferred cofactor and monitored every 1015 s for production of reduced cofactor at A 340nm using a microplate reader (Tecan Genios)

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