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To achieve each niclosamide concentration, the 30mM stock solution was made by weighing 100.09mg of AK Sci niclosamide powder (i.e., 98.13mg of niclosamide, allowing for the 2% impurity that is filtered out later) and dissolving in ethanol up to 10mLs total volume

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A subpopulation of macrophages infiltrates hypertrophic adipose tissue and is activated by free fatty acids via Toll-like receptors 2 and 4 and JNK-dependent pathways