2009 Jul;8(8):697-701
For antibody labeling, after treatments at 37 C, cells were placed on ice to arrest further endocytosis before fixation, and the surface GLP-1R was detected by ELISA with monoclonal anti-human GLP-1R antibody 68 (Mab 3F52, Developmental Studies Hybridoma Bank (DSHB), 1/100) plus horseradish peroxidase (HRP)-conjugated rabbit anti-mouse secondary (ab97046, Abcam, 1/5,000)
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High-dose supplements with beta-carotene (either alone or with vitamins E or A) , do not reduce the risk of cancer or cardiovascular disease and may even be harmful to smokers or former asbestos workers who are vulnerable to an excess of vitamin A
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Recent animal studies also suggest that GLP-1s may provide protection from Alzheimer's and age-related cognitive decline