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2.2 Fluorescence in situ hybridization and whole-exome sequencing and mutation calling Interphase fluorescence in situ hybridization (FISH) was performed on formalin-fixed paraffin-embedded (FFPE) sections using a MDM2 dual color break-apart probe at 12q15 and CDK4 dual color break-apart probe at 12q14 (Linked-Biotech Pathology, Guangzhou, China), respectively

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Along the vertical insertion path, a slight sensation of resistance breakthrough will be felt when the penetrates the bottom of the dermal layer (about 3 mm deep)