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Once cells were proliferating, they were moved up to higher cell volumes and adapted to DMEM cell culture media with 20% dFBS, 50 U ml 1 penicillin and streptomycin and 1 mM sodium pyruvate for at least 1 week before experimental setup

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Samples were obtained at sequential time points after the addition of 2-deoxyglucose (2-DG) and diamide

Volterra A, Meldolesi J

Carnitine concentration determination After the feeding trial, the whole liver and parts of muscle of 4 fishes collected from each group were weighted and homogenized by pure-water (1:10, w/v) by using a drill-driven Teflon glass homogenizer (Scientz Biotec, China) with 34 strokes

Zwaszcza ci, ktrzy uywaj wyszych dawek, poczuj przyspieszenie budowy mini, popraw oglnego stanu zdrowia oraz kondycji