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For microscopic analysis, 2 10 5 T-cells were initially allowed to adhere on coated coverslips and immunocytochemistry protocols were performed as described ( Kinetic trapping by Trx1 The Trx1 trapping mutant (Trx1 C35S) was purified and loaded on streptavidin beads as described ( 2 O 2 for 10 min at RT