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Biochemical assays Blood was collected from the abdominal aorta at 0, 4, 20, and 44 hours post-CLP

Human randomized controlled trial data are comparatively thin

5 million cells were used for the GSH assay after lysing by sonication (20 kHz, 70% amplitude, 2 10 s)

A deficiency of vitamin B12 can present as a mix of neurologic symptoms, including paresthesias, loss of proprioception, or autonomic nervous system dysfunction, and hematologic abnormalities, such as macrocytic anemia, thrombocytopenia, or pancytopenia (3)

Iron accumulation [12] and lipid peroxidation [13, 14], two ferroptosis hallmarks, have been detected during early AD pathology

RNA sequencing Total RNA was isolated from the Achilles tendon using the ISOGEN reagent (Nippon Gene, Tokyo, Japan)