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Samples were incubated with anti-GPX4 antibody (1:400, BOSTER, #BA3802-1) overnight at 4 C and subsequently rinsed with PBS

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During initiation, cells export reduced GSH through plasma membrane transporters before membrane rupture, establishing a redox environment permissive for apoptotic signaling

Haas MJ, Jafri M, Wehmeier KR, Onstead-Haas LM, Mooradian AD (2016) Inhibition of endoplasmic reticulum stress and oxidative stress by vitamin D in endothelial cells