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Preclinical models found that GLP-1 RAs reduce intestinal inflammation in dextran sulfate sodium (DSS)-induced colitis, by increasing IL-22 production by colonic IELs and several beneficial bacteria, including Firmicutes, Proteobacteria and Lactobacillus reuteri (93)

The triple-incretin pharmacology represents an attempt to combine the insulin-sensitizing and appetite-modulating effects of GLP-1/GIP agonism with the energy-expenditure effects of glucagon receptor activation

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The final reaction comprises of 2 ml of Na-phosphate buffer (pH 7.3, 0.5 M,) 0.1 ml of enzyme extract, 0.1 ml of EDTA (3 mM) and 0.1 ml of H 2 O 2 (3 mM) for 5 min

Clinical research suggested doses in the range of 1-10mcg per kilogram of body weight showed efficacy, though most protocols standardized around 4-6mcg/kg for practical application