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In order to collect a sufficient number of cells for implantation, isolated chondrocytes usually undergo an extensive expansion from 0.10.2 million to 4060 million cultured chondrocytes gradually lose the proliferative capacity and the potential of generating the cartilage-specific matrix, a phenomenon known as dedifferentiation

since 2005 and that altogether nearly 11.6 billion records have been breached

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The development of 3D culture models and organ-on-chip platforms brought a degree of complexity to classic in vitro for more physiological models of organs including the BBB