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On the day of the experiment, they were equilibrated for 3 hours in serum-free DMEM containing 5.6 mM glucose, penicillin, streptomycin, and 0.1% BSA

poorly controlled diabetes during pregnancy increases the maternal risk for diabetic ketoacidosis, preeclampsia, spontaneous abortions, preterm delivery, and delivery complications and increases the fetal risk for major birth defects, stillbirth, and macrosomia related morbidity

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The combined treatment with AA and GSE demonstrated a potent antitumor effect against solid tumors, characterized by widespread areas of complete necrosis, residual tumor cells, cystic structures surrounded by fibrous tissue, and a markedly reduced number of viable tumor cells ( Figure 7 , Table 3 )

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