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The protein-bound dye was dissolved in 150 L/well of 10 mM Tris base (pH: 10.0), and the plate was shaken on a shaker at approximately 150 rpm for at least 10 min

A peptide turns cloudy or gels when its molecules aggregate, meaning they stick to each other instead of spreading evenly through the solvent

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At present, even though the comparative efficacy of tirzepatide vs these higher dosing regimens of dulaglutide and semaglutide is unknown, available data suggest that tirzepatide could be a reasonable treatment option when glycaemic control and body weight loss are therapeutic priorities