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After collagen treatment of the lesion-containing material and sieving, the washed cells were layered over a five-layer discontinuous Nycodenz gradient of 0.5, 1.0, 5.0, 10 and 30% (w/v) Nycodenz and centrifuged at 1200 g for 15 min at 10C [13,14]

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5.3.2 Offering therapy based on tumour microenvironment (TME) The surrounding tissue environment has been particularly shown to affect various processes in lipid peroxidation and response to treatment

Suspected side effects should be reported to the MHRA Yellow Card scheme in addition to informing the clinical trial team