Western blotting (WB) Whole-cell proteins were extracted from GC tissues and cells with RIPA lysis buffer (P0013B, Beyotime, China) supplemented with PMSF (P0100, Solarbio, China) and a protein phosphatase inhibitor (P1260, Solarbio, China)
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Responsiveness to forceps stimuli in rats (64) (scored 05, min/med/max) that received cuprizone diet, and then cuprizone intragastrical applications, and thereby, cuprizone toxicity manifestations
They act on different receptors, through different pathways, with different effects on the growth-hormone pulse which is precisely why they're so often studied together rather than chosen one over the other
