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Second, proline substitutions at positions 25, 28, and 29 reduce the tendency to form beta-sheet structures that lead to aggregation

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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore