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Four independent transfections were carried out using a plasmid designed to disrupt the pbgst gene by double crossover recombination (Figures 1A,C)

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Notably, GHK-Cu levels in circulation are known to decline with age, a pattern that has fueled substantial research interest in the peptide's potential relevance to tissue aging and regenerative capacity over the lifespan

J Biol Chem 281:40354 Yang Y, Loscalzo J (2005) S -nitrosoprotein formation and localization in endothelial cells

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