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Before imaging, islets were loaded with 8 M Fluo-4 AM (Thermo Fisher) at room temperature (21C) for 90 minutes in imaging buffer consisting of 140 mM NaCl, 3.6 mM KCl, 0.5 mM MgSO 4 , 1.3 mM CaCl 2 , 10 mM HEPES (pH 7.4 with NaOH), 5 mM NaHCO 3 , and 0.5 mM NaH 2 PO 4 with either 5.5 mM or 16.7 mM glucose (reflecting the culture conditions), 1 mg/mL BSA, and 0.02% pluronic F-127 (Thermo Fisher)

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10.3390/ijms23052758 25 FelembanA

Brain cell metabolism was preserved in one trial, and the other found reduced amyloid-beta in extracellular vesicles, but neither trial found that GLP-1 drugs reduced the build-up of amyloid-beta in the brain or helped stave off cognitive decline

Lab work through Quest and Tasso is included but not required for every patient

Um, it's also hypothesized to increase ni uh, nitric oxide, which is a, uh, blood vessel dilator, uh, improving microcirculation