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CDK11 and cyclin L-SAP30BP for pull-down assays An expression construct encoding Twin-Strep-tagged CDK11B p58 (CDK11B p110 residues 357795) was cloned and used to prepare bacmids, which were then transfected into Sf9 insect cells (Thermo Fisher Scientific)

A slowdown after the early weeks is normal and expected, not a sign that something is wrong

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If rapid pain relief is required, this minor delay is unlikely to pose problems for most individuals

It's the single most predictive factor for the MT-1 timeline