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For expression in yeast, these sequences were then transferred to pYES-DEST52 vector (Invitrogen, USA) through Gateway cloning technology using LR clonase-II enzyme following the manufacturers instructions

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doi: 10.1158/1078-0432.Ccr-16-1647 67 PlewaSHoraaADereziskiPKlupczynskaANowak-MarkwitzEMatysiakJet al

Ramakrishnan, A