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GSH is manufactured in the cytosol by a two-step process: the first step, which combines cysteine and glutamate, is catalyzed by -glutamylcysteine synthetase (GCS)

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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

10.1002/mnfr.202200275 169 WangX.ZhangM.MaoC.ZhangC.MaW.TangJ.et al (2023d)

[DOI] [PMC free article] [PubMed] [Google Scholar] 122.Hsieh M.J., Liu H.T., Wang C.N., Huang H.Y., Lin Y., Ko Y.S., Wang J.S., Chang V.H., Pang J.S