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Importantly, high-dose regimensparticularly bolus or intermittent administrationhave not demonstrated clear clinical benefits in randomized controlled trials [271, 272].On the contrary, several studies have suggested that such strategies may increase the risk of adverse outcomes, including falls [273, 274]

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Such haptenated proteins can disrupt protein folding or function and lead to direct toxicity, or can be recognized as non-self leading to immune-mediated toxicity (Tafazoli and OBrien, 2009

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Collectively, this study provides a solid foundation for future exploration into the impact of NNMTi-and diet-mediated changes to the gut microbiome in obese and lean populations, as well as future targets to investigate mechanistically with NNMTi treatment