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Most people who have taken GLP-1 drugs say they got them from one of their doctors (76%), while about one in six (17%) say they got them from an online provider or website, and one in ten (9%) report getting them from a medical spa or aesthetic medical center

Subcutaneous tissue and muscles were separated by blunt dissection with forceps, the dura mater was blotted dry with a sterile cotton swab, and CSF was collected using a sterile pulled capillary to penetrate the cisterna magna (through the dura mater, lateral to the arteria dorsalis spinalis) and immediately snap-frozen in liquid nitrogen and stored at 80 C

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All the cells were cultured in DMEM (Invitrogen