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The other 7 bullfrogs were injected into 1.5 mL centrifuge tubes with a 1 mL syringe, and then centrifuged at 4C for 10 min (12000 r/min, 4C) after 24 h

CMAP was recorded after stimulating the first and second electrodes followed by recording of the amplitude, proximal and distal latencies, and polyphasic changes

10.2337/db07-0075 11 AnnaA.Mara-JosM.AidaS.MayteB.MontserratP

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This dual role of ubiquitination in both potentially targeting ChaC1 for degradation and stabilizing it highlights a nuanced regulatory mechanism where the context and perhaps the nature of the ubiquitin linkages or chains may dictate the ultimate fate of the ChaC1 protein

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