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Subsequently, the dorsal skin was separated using a scalpel and homogenized in RNA lysate reagent (LS1040, Promega) with the assistance of Gentle MACSTM Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany)

METHODS An electronic search was conducted from June 2024 to July 2024, with an updated search in August 2024, using databases PubMed, PubMed Central, Clinicaltrials.gov, Ovid Medline, and CINAHL

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1C), indicating dose-dependent excitatory effects