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the slices were deparaffinized and stained with hematoxylin a After deparaffinization, the slices were stained with hematoxylin and eosin (H.E.), and the slices were sealed and observed and photographed with an orthogonal fluorescence microscope (Leica DM5500B)

The non-12a-OH BAs in the tissues of mice were calculated by summing the values of BA species, including MCA, MCA, UDCA, HDCA, CDCA, LCA, T-MCA, T-MCA, TUDCA, THDCA, TCDCA, TLCA, GUDCA, GCDCA, GLCA

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