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The final concentration will depend on the specific requirements of the research protocol
Briefly, a mixed standard solution consisting of the four cobalamins, prepared by mixing water (150 L), CNCbl (100 L of ~0.1 mg/mL), OHCbl (250 L of ~0.1 mg/mL), AdoCbl (250 L of ~0.1 mg/mL), and MeCbl (250 L of ~0.1 mg/mL), was measured using HPLC-DAD
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39 Consequently, patients should be monitored with FBE, LFTs and measurement of levels of thiopurine metabolites