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The RT-qPCR reaction volume was 20 L, which included 10 L 2 SYBR Premix Ex Taq (Takara Bio, Inc., Kusatsu, Japan), 1 L (10 pmol/L) forward primer, 1 L (10 pmol/L) reverse primer, 2 L template DNA and 6 L sterile water, primer sequence as shown in Table 1

For example, if youre going on vacation from Sunday until Tuesday, inject on Tuesday to maintain the necessary 7-day interval

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