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The technique separates compounds based on hydrophobicity and molecular size, producing a chromatogram showing: Main peak purity : The percentage of total UV-absorbing material represented by the target peptide peak (should be 98% for research-grade material) Deletion sequences : Truncated peptides missing one or more amino acids, appearing as earlier-eluting peaks Insertion sequences : Longer-than-expected peptides from synthesis errors, appearing as later-eluting peaks Diastereomers : Peptides with incorrect amino acid stereochemistry, often appearing as shoulder peaks adjacent to the main peak Arma Peptides supplies BPC-157 5mg and BPC-157 10mg with batch-specific HPLC chromatograms showing 99% purity
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doi: 10.1186/s13046-025-03361-3 65 HuangSPChanYCHuangSYLinYF
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