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Monolayers of immortalized mouse brain endothelial cells (bEnd.3) were treated with recombinant TGF 1 (rTGF 1) and permeability to fluorescein isothiocyanate-dextran (FITC-dextran), MMP9 and claudin-5 expression was assessed

There's a lot to know because hunger is tied in with physiological processes, as well as psychology and behavior