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(visit source) Dawczynski C, Martin L, Wagner A, Jahreis G

Osteoporosis Untreated hyperthyroidism also can cause brittle bones, or osteoporosis, because too much thyroid hormone interferes with your body's ability to incorporate calcium into your bones

doi: 10.1007/BF01225454 2 CreutzfeldtW

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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice