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23 Placenta-derived EVs enter the maternal circulation as early as six weeks of gestation, and their concentration increases with gestational age

They overlap with trial findings but reflect a different exposure pattern (lower per-dose, daily, longer duration)

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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight

Song Y et al