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Roberts DM, Heilmair R, Buckley NA, et al

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TNF, perforin, granulysin and granzyme A showed a downward trend in 1% oxygen but this was not significant when compared to vehicle treated cells (Extended Data Fig

3 (9), e1701028

It acts as a protective antioxidant, such as neutralization of stress responses, modulation of antibiotic targets, and interference with antibiotic activation

Afterwards, cells were washed with PBS, and incubated at room temperature for 30 min with an anti-rabbit IgG Alexa Fluor 546 antibody (1:100, #A-11035, Thermo Fisher Scientific, Waltham, MA, USA)