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Shin D, Lee J, You JH, Kim D, Roh J-L

Although AAVs have traditionally been the main vector used to deliver CRISPR-Cas9 constructs, the relatively small packaging size (~4.5 kb) makes it so that researchers must deliver two separate vectors containing the CRISPR-Cas9 construct (~4.2 kb) and gRNA separately

The cohort included 82% males and 18% females, and children with genetic syndromes, prematurity, major medical disease or severe irritability were excluded, although common comorbidities such as gastrointestinal, allergic, neurological, and sleep disorders were present

Thus, enriched G- in patients with GER symptoms may induce esophageal barrier dysfunction via the LPS-TLR2-IL-6-claudin-1-DIS pathway (48)