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For brain homogenates, 0.2 ml of the preparation was diluted to 1 ml by the respiration buffer

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RNA extraction was performed 24 h post-transfection, while protein extraction was carried out 48 h post-transfection for subsequent experiments

Chlorophyll degradation was less than the 90% loss described for broccoli stored at 20C for 5 days (Wang et al., 2024c)