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Intracellular GSH/GSSG measurement The GSH and GSSG levels in whole liver tissues or cultured cells were measured using OxiSelect Total Glutathione (GSSG/GSH) Assay Kits in accordance with the manufacturers instructions (Cell Biolabs, San Diego, CA, USA)

Effects of a novel ascorbate-based protocol on infarct size and ventricle function in acute myocardial infarction patients undergoing percutaneous coronary angioplasty

JAMA 331(19):16721673

Enhancing Sperm Motility and Count For sperm to reach an egg, they must be highly motile

Centrifuge at 8,000 g for 10 min at 4C