The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay
For the myotendinous junction, as a combination point of anatomical and structural particularities (largely reviewed in [124,125]) such a new therapy possibility might be particularly important given the still unresolved problem of being the weakest element in the muscletendon unit, (and bonetendon junctions as extremely specialized tissues in general)
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