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Genetic knockout of solute carrier 22A member 4 (SLC22A4), part of the large solute carrier 22A (SLC22A) family, in both mice (25) and zebrafish (26) , resulted in dramatic reduction of ergothioneine to undetectable concentrations in many tissues (e.g

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Isotope-labeling studies further confirmed that NRH is completely incorporated into NAD +, with nicotinamide mononucleotide hydrogen (NMNH) serving as a key intermediate

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