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Insulin should be injected into the fatty tissue just below your skin

Negative controls were treated either with pre-immune serum instead of the primary antibody (Supplementary Figure 1A) or non-specific secondary antibody (goat anti-mouse IgG) (Supplementary Figure 1B)

Water retention is significant enough to be visible or uncomfortable

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Yeah, but maybe that was a different time

Some GPs may incorrectly believe that only patients with a definite diagnosis of PA, those with a positive intrinsic factor antibody test, require treatment for life so seem to be using this as their treatment exclusion criteria