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If necessary, keep the supernatant for analysis

The free GHK tripeptide was first isolated from human plasma in 1973 by Loren Pickart, and the copper-bound form has been studied since the 1980s as a regenerative-research reference compound.nnActive research areas include extracellular matrix gene expression, collagen and decorin regulation, matrix-metalloproteinase modulation, antioxidant pathway activity, and copper-mediated cell signalling

Thus, doxorubicin, a potent anticancer drug, but with heavy side effects, was delivered in association with chemically immobilized bromelain, by lactobionic acid-modified chitosan NPs

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