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doi: 10.3389/fcell.2025.1718643 Received 04 October 2025 Revised 05 November 2025 Accepted 10 November 2025 Published 21 November 2025 Volume 13 - 2025 Edited by Carol Margaret Trim, Canterbury Christ Church University, United Kingdom Reviewed by Juliano C

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Calorie restriction in overweight males ameliorates obesity-related metabolic alterations and cellular adaptations through anti-aging effects, possibly including AMPK and SIRT1 activation

Patients typically report increased mental clarity, reduced fatigue, and a sense of physical lightness early in the protocol

Maintain a Balanced Diet A balanced diet rich in B12-rich foods can help maintain optimal B12 levels

Shubin, A