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In a typical encapsulation experiment, PLGA nanoparticles exhibited a hydrodynamic diameter of 170 30 nm (z-average, PDI = 0.162) as measured using dynamic light scattering with anionic effective surface charge (20.5 5.3 mV) due to the terminal carboxylate groups on the PLGA polymers

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That is a mean difference of 9.1 fewer headache days per month, a reduction that reached statistical significance with a p-value less than 0.001

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Sinha N, Whelan EC, Tobias JW, Avarbock M, Stefanovski D, Brinster RL (2021) Roles of Stra8 and Tcerg1l in retinoic acid induced spermatogonial differentiation in mouse