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Popcorn pellets were collected by centrifugation for 30 min at 4000 g, resuspension in 100 L of ice-cooled lysis buffer [50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 , 0.5% NP40 (Research Products International), 125 U/mL Benzonase (Millipore), 1x protease inhibitor cocktail (Roche, cOmplete ULTRA tablets, mini, EDTA-free), 0.5 mg/ml lysozyme (Fisher Scientific)], followed by dropwise addition into liquid nitrogen and stored at 80 C

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The pyridoxine formulation may be particularly beneficial for this age group, addressing both the increased nausea susceptibility and the higher nutritional needs simultaneously