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RNA (1.5 g) was used for cDNA library preparation with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, NEB) following the manufacturers protocol

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Hence, intracellular Ca2+ mediates both mechanisms of ALAs apoptotic effects [86]

Both PCR products were cloned into pCAP s vector (Roche, www.roche-applied-science.com) for sequence confirmation

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This early success is incredibly encouraging and helps many feel motivated to continue and reach their goals