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Recruitment was quantified during 30 min as 535 nm over 460 nm fluorescence ratio in live cells at 37 C using FlexStation3 and the SoftMaxPro 5 software (Molecular Devices)

On any U-100 syringe the scale is fixed: 1.0 mL is 100 units, regardless of whether the barrel is a 0.3, 0.5, or 1 mL syringe

Blood was centrifuged in a standard centrifuge (Labofuge 300, Heraeus Holding, Germany) at 3000 g 10 min

& Hilario, E

The frequency of each modification introduced was monitored by LCMS analysis