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In addition to MTT assays, we also performed cell counting with a Countess Automated Cell Counter (Applied Biosystems) in experiments where we treated primary melanoma cell lines WM115 and WM1862 with the EPAC inhibitor ESI-09 and transfected with EPAC siRNA

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Minocycline prevents nigrostriatal dopaminergic neurodegeneration in the MPTP model of Parkinsons disease

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