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The sulfonic acid replacement by a carboxylic acid ( 34 ) maintained a good solubility but led to a substantial loss of binding activity in the CPMG assay (Table 1)

pylori cells were harvested by centrifugation (7000 g at 4C for 10 min), washed in 20 ml pre-chilled buffer (0.05 M Tris pH 8.22 mM EDTA) and suspended in 10 ml of the same buffer

Allow at least 15 to 30 minutes if the vials have been in a -20C freezer

BPC 157 cng gip tng tc qu trnh cha lnh cc m mm