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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100

Chang and colleagues (J Appl Physiol, 2011, PMID 21030672) reported increased FAK and paxillin phosphorylation in cultured rat tendon fibroblasts

However, the peptide had minimal side effects in mice.